Keep nibbling at the edges.
نویسنده
چکیده
Alifelong passion for natural history and an aptitude for chemistry had led me to study for a B.Sc. in biochemistry at University College London (UCL) from 1963 to 1966. It was during this period that the allosteric theory of enzyme regulation was put forward by JacquesMonod, Jean-Pierre Changeux, and Francois Jacob at the Institute Pasteur. This explained how the end products of a biosynthetic pathway exerted feedback control on the enzyme catalyzing the initial and rate-limiting step. Reading these papers sparked an interest in enzyme regulation and led me to become a graduate student in the UCL laboratory of Michael Rosemeyer, with whom I had carried out a short undergraduate project the previous year.Michael had offered a project to study the control of glucose-6-phosphate dehydrogenase, the first enzyme in the pentose phosphate shunt. I purified Glu-6-P dehydrogenase and determined its subunit composition (1, 2) because the “quaternary structure” of an enzyme was thought to be key to how it was regulated by allosteric effectors. The project gave me an excellent grounding in protein chemistry, but Glu-6-P dehydrogenase seemed merely to be regulated by the NADPH/NADP ratio, and it was clear that I needed to find a more challenging problem to work on. The idea of applying to Edmond (Eddy) Fischer for a postdoctoral position arose during a discussion with Prakash Datta, a professor in the department at UCL, and BillWhelan, the Chairman of Biochemistry at the University of Miami. Bill had just founded FEBS Letters and had come to visit because Prakash had become the firstManaging Editor of the journal. I had heard Eddy Fischer give several lectures at a protein chemistry summer school in Venice in 1967, and so Bill, who was a friend of Eddy, agreed to write a letter of support to accompany my application. However, my initial approaches were unsuccessful, and only after I had obtained a UK Science Research Council (subsequently renamed the Biotechnology and Biological Sciences Research Council) postdoctoral fellowship funded by NATO did Eddy agree to let me come. To this day, I therefore always re-evaluate very carefully persistent postdoctoral applicants who will not take no for an answer!
منابع مشابه
The effect of meal frequency on serum immunoglobulin profile and insulin in rat
Background: Although consumption of three or less than three meals per day (gorging regimen) is usual in some communities, in western population there is an inclination towards many small meals a day (nibbling regimen). Up to now the effects of meal frequency on serum immunoglobulins have not been investigated in laboratory animals. There is also some controversy information about the effect of...
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Objective Despite numerous reports on the effects of meal frequency on biochemical parameters such as plasma lipid profile, glucose and insulin, there is almost no study to investigate the influence of meal frequency on immune system. In the present article, for the first time, the effect of meal frequency on complement components C3 and C4, as key components of the complement system, has been...
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Monocyte-derived dendritic cells (DC) possess the unique capacity to capture Ag from live cells through intimate cell contact, a process referred to as nibbling. We sought to define the receptor(s) mediating DC nibbling. Uptake of fluorescently labeled plasma membrane from live cells by DC was inhibited by protease treatment and by a panel of polyanionic ligands, implicating scavenger receptors...
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Six grossly obese patients were fed 5000 calorie diets for 4 wk. During one period of 2 wk, the calories were consumed over 4 hr (gorging) and during the other 2 wk, the dietary intake was spread over 20 hr (nibbling). Each of these periods followed a low caloric intake which lasted at least 10 days. Three male patients (group I) were studied at or near their maximal weight and three females (g...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 284 36 شماره
صفحات -
تاریخ انتشار 2009